Links

  • 2002:  PhD in Synthetic Organic Chemistry, University of Bologna, IT
  • 2002 – 2005:  Postdoc, University College Dublin, IE
  • 2006 – 2013:  Lecturer, University College Dublin, IE 
  • 2014 – 2016:  Senior Lecturer, University College Dublin, IE
  • 2016 – 2019:  Associate Professor in Biocatalysis, University of Nottingham, UK  &  Adjunct Professor, UCD, IE
  • since 2019:  Professor of Sustainable Pharmaceutical Chemistry, University of Bern, CH

We have a keen interest in the development of alternative biocatalysts for synthetic and industrial applications. Predominantly our research focuses on the discovery, characterization and evolution of enzymes originating from extremophilic organisms, more specifically salt adapted microbes which can withstand high salt concentrations. Currently we are working with a variety of enzymes encompassing oxido-reductases, transaminases, and hydrolases. The environmental adaptation of the organisms translates into unique enzymatic properties such as high tolerance to organic solvents, broad substrate scope, and remarkable stability.

To further extend enzymatic stability we have developed tailored immobilization strategies which allow us to use (and re-use) the enzymes in batch as well as in flow-systems. Flow-biocatalysis is currently our main tool in the synthesis of valuable molecules.

Our research is highly interdisciplinary with a number of collaborators in the US as well as Europe, and together we are looking at the assembly of semi-synthetic enzymes with enhanced biocatalytic properties.

We have a smaller synthetic stream in our lab which is dedicated to the incorporation of rigid, non-natural amino acids into peptidomimeticts which have demonstrated superior anti-cancer activity.

For a full list of publications, please see:

Researcher ID: E-1786-2012

ORCID: 0000-0003-1704-0642

 

 

Publications recorded in BORIS (Bern Open Repository and Information System): 

Number of items: 36.

Journal Article

Montanari, Ulisse; Cocchi, Davide; Brugo, Tommaso Maria; Pollicino, Antonino; Taresco, Vincenzo; Romero Fernandez, Maria; Moore, Jonathan C.; Sagnelli, Domenico; Paradisi, Francesca; Zucchelli, Andrea; Howdle, Steven M.; Gualandi, Chiara (2021). Functionalisable Epoxy-rich Electrospun Fibres Based on Renewable Terpene for Multi-Purpose Applications. Polymers, 13(11), p. 1804. MDPI 10.3390/polym13111804

Roura Padrosa, David; Nisar, Zoya; Paradisi, Francesca (2021). Efficient Amino Donor Recycling in Amination Reactions: Development of a New Alanine Dehydrogenase in Continuous Flow and Dialysis Membrane Reactors. Catalysts, 11(4), p. 520. MDPI 10.3390/catal11040520

Heckmann, Christian M.; Dominguez, Beatriz; Paradisi, Francesca (2021). Enantio-Complementary Continuous-Flow Synthesis of 2-Aminobutane Using Covalently Immobilized Transaminases. ACS sustainable chemistry & engineering, 9(11), pp. 4122-4129. American Chemical Society 10.1021/acssuschemeng.0c09075

Roura Padrosa, David; Marchini, Valentina; Paradisi, Francesca (2021). CapiPy: python based GUI-application to assist in protein immobilization (In Press). Bioinformatics Oxford University Press 10.1093/bioinformatics/btab030

Delgado, Lidia; Heckmann, Christian M.; Di Pisa, Flavio; Gourlay, Louise; Paradisi, Francesca (2021). Release of Soybean Isoflavones by Using a β‐Glucosidase from Alicyclobacillus herbarius. ChemBioChem, 22(7), pp. 1223-1231. Wiley-VCH 10.1002/cbic.202000688

Delgado, Lidia; Heckmann, Christian M.; De Benedetti, Stefano; Nardini, Marco; Gourlay, Louise J.; Paradisi, Francesca (2021). Producing natural vanilla extract from green vanilla beans using a β-glucosidase from Alicyclobacillus acidiphilus. Journal of biotechnology, 329, pp. 21-28. Elsevier 10.1016/j.jbiotec.2021.01.017

Benítez-Mateos, Ana I.; Contente, Martina L.; Roura Padrosa, David; Paradisi, Francesca (2021). Flow biocatalysis 101: design, development and applications. Reaction chemistry & engineering, 6(4), pp. 599-611. Royal Society of Chemistry 10.1039/D0RE00483A

Romero-Fernandez, Maria; Paradisi, Francesca (2021). Biocatalytic access to betazole by one-pot multienzymatic system in continuous flow. Green chemistry, 23(12), pp. 4594-4603. Royal Society of Chemistry 10.1039/D1GC01095F

Benítez-Mateos, Ana I.; Bertella, Stefania; Behaghel de Bueren, Jean; Luterbacher, Jeremy S.; Paradisi, Francesca (2021). Dual Valorization of Lignin as a Versatile and Renewable Matrix for Enzyme Immobilization and (Flow) Bioprocess Engineering. ChemSusChem, 14(15), pp. 3198-3207. Wiley-VCH 10.1002/cssc.202100926

Planchestainer, Matteo; McMaster, Jonathan; Schulz, Christine; Paradisi, Francesca; Albrecht, Martin (2020). Carbene‐Induced Rescue of Catalytic Activity in Deactivated Nitrite Reductase Mutant. Chemistry - a European journal, 26(66), pp. 15206-15211. Wiley-VCH 10.1002/chem.202002444

Hegarty, Eimear; Paradisi, Francesca (2020). Implementation of Biocatalysis in Continuous Flow for the Synthesis of Small Cyclic Amines. CHIMIA, 74(11), pp. 890-894. Schweizerische Chemische Gesellschaft 10.2533/chimia.2020.890

Contente, Martina L.; Fiore, Noemi; Cannazza, Pietro; Roura Padrosa, David; Molinari, Francesco; Gourlay, Louise; Paradisi, Francesca (2020). Uncommon overoxidative catalytic activity in a new halo‐tolerant alcohol dehydrogenase. ChemCatChem, 12(22), pp. 5679-5685. WILEY-VCH 10.1002/cctc.202001112

Heckmann, Christian M.; Gourlay, Louise J.; Dominguez, Beatriz; Paradisi, Francesca (2020). An (R)-Selective Transaminase From Thermomyces stellatus: Stabilizing the Tetrameric Form. Frontiers in Bioengineering and Biotechnology, 8(707) Frontiers Media 10.3389/fbioe.2020.00707

Roura Padrosa, David; Benítez-Mateos, Ana I.; Calvey, Liam; Paradisi, Francesca (2020). Cell-free biocatalytic syntheses of L-pipecolic acid: a dual strategy approach and process intensification in flow. Green chemistry, 22(16), pp. 5310-5316. Royal Society of Chemistry 10.1039/D0GC01817A

ul Haque, R; Allers, T; Paradisi, Francesca (2020). Haloferax volcanii for biotechnology applications: challenges, current state and perspectives. Applied microbiology and biotechnology, 104(4), pp. 1371-1382. Springer 10.1007/s00253-019-10314-2

Contente, Martina Letizia; Tamborini, Lucia; Molinari, Francesco; Paradisi, Francesca (2020). Aromas flow: eco-friendly, continuous, and scalable preparation of flavour esters. Journal of flow chemistry, 10(1), pp. 235-240. Springer 10.1007/s41981-019-00063-8

Almulhim, Nouarh; Moody, Nicholas R.; Paradisi, Francesca (2020). Engineering novel S-glycosidase activity into extremo-adapted β-glucosidase by rational design. Applied microbiology and biotechnology, 104(10), pp. 4407-4415. Springer 10.1007/s00253-020-10582-3

Delgado, Lidia; Parker, Mango; Fisk, Ian; Paradisi, Francesca (2020). Performance of the extremophilic enzyme BglA in the hydrolysis of two aroma glucosides in a range of model and real wines and juices. Food Chemistry, 323, p. 126825. Elsevier 10.1016/j.foodchem.2020.126825

Romero-Fernández, María; Paradisi, Francesca (2020). Protein immobilization technology for flow biocatalysis. Current opinion in chemical biology, 55, pp. 1-8. Elsevier 10.1016/j.cbpa.2019.11.008

Heckmann, Christian M.; Paradisi, Francesca (2020). Looking back: A short history of the discovery of enzymes and how they became powerful chemical tools. ChemCatChem, 12(24), pp. 6082-6102. WILEY-VCH 10.1002/cctc.202001107

Contente, Martina L.; Roura Padrosa, David; Molinari, Francesco; Paradisi, Francesca (2020). A strategic Ser/Cys exchange in the catalytic triad unlocks an acyltransferase-mediated synthesis of thioesters and tertiary amides. Nature catalysis, 3(12), pp. 1020-1026. Nature Publishing Group 10.1038/s41929-020-00539-0

Roura Padrosa, David; Alaux, Raphael; Smith, Phillip; Dreveny, Ingrid; López-Gallego, Fernando; Paradisi, Francesca (2019). Enhancing PLP-Binding Capacity of Class-III ω-Transaminase by Single Residue Substitution. Frontiers in Bioengineering and Biotechnology, 7(282) Frontiers Media 10.3389/fbioe.2019.00282

Contente, Martina Letizia; Farris, Stefano; Tamborini, Lucia; Molinari, Francesco; Paradisi, Francesca (2019). Flow-based enzymatic synthesis of melatonin and other high value tryptamine derivatives: a five-minute intensified process. Green chemistry, 21(12), pp. 3263-3266. Royal Society of Chemistry 10.1039/C9GC01374A

Planchestainer, Matteo; Hegarty, Eimear; Heckmann, Christian M.; Gourlay, Louise J.; Paradisi, Francesca (2019). Widely applicable background depletion step enables transaminase evolution through solid-phase screening. Chemical Science, 10(23), pp. 5952-5958. The Royal Society of Chemistry 10.1039/C8SC05712E

Contente Martina Letizia, Contente; Paradisi, Francesca (2019). Transaminase-catalyzed continous synthesis of biogenic aldehydes. ChemBioChem, 20(22), pp. 2830-2833. Wiley-VCH 10.1002/cbic.201900356

Planchestainer, Matteo; Ségaud, Nathalie Sylvie Laetitia; Shanmugam, Muralidharan; McMaster, Jonathan; Paradisi, Francesca; Albrecht, Martin (2018). Carbene in Cupredoxin Protein Scaffolds: Replacement of a Histidine Ligand in the Active Site Substantially Alters Copper Redox Properties. Angewandte Chemie (International ed.), 57(33), pp. 10677-10682. Wiley-VCH 10.1002/anie.201807168

Book Section

Romero Fernández, María; Paradisi, Francesca (2020). General overview on immobilisation techniques of enzymes for biocatalysis. In: Benaglia, Maurizio; Puglisi, Alessandra (eds.) Catalyst Immobilization: Methods and Applications (pp. 409-436). Wiley-VCH

Conference or Workshop Item

Paradisi, Francesca (3 March 2020). Progress in Flow Biocatalysts: Multi-enzyme Cascades and Implementation (Unpublished). In: Flow Chemistry Europe 2020.

Paradisi, Francesca (17 February 2020). Versatility of transaminases in flow biocatalysis (Unpublished). In: Amine Biocatalysis 4.0.

Planchestainer, Matteo; Lenzen, Karst Eelco; Paradisi, Francesca; Albrecht, Martin (6 December 2019). Installing a metal active centre in peptide scaffolds thought an unnatural NHC-amino acid (Unpublished). In: CSCB Symposium. Dublin, Ireland. 6.12.2019.

Paradisi, Francesca (6 December 2019). Heterogenous biocatalysis in continuous flow systems as a new approach to synthetic chemistry (Unpublished). In: Recent Advances in Synthesis and Chemical Biology XVIII. Dublin, Ireland.

Paradisi, Francesca (21 November 2019). Biocatalysis and Flow Technology (Unpublished). In: Swiss Industrial Biocatalysis Consortium 2019.

Paradisi, Francesca (21 June 2019). Continuous Flow biocatalysis for highly sustainable and versatile syntheses (Unpublished). In: 71st Irish Universities Chemistry Research Colloquium.

Planchestainer, Matteo; Ségaud, Nathalie Sylvie Laetitia; Paradisi, Francesca; Albrecht, Martin (14 June 2019). Carbene- rather than Nitrogen-metal bonding mode alters metallo-enzymes features (Unpublished). In: NoNoMeCat Symposium. Basel, Switzerland. 14.6.2019.

Planchestainer, Matteo; Lenzen, Karst Eelco; Paradisi, Francesca; Albrecht, Martin (9 June 2019). Installing a metal active centre in peptide scaffolds thought an unnatural NHC-amino acid (Unpublished). In: Swiss Chemical Society Fall Meeting 2019. Zurich, Switzerland. 6.9.2019.

Paradisi, Francesca (19 May 2019). Sustainable Catalysis: enzyme-mediated synthesis of high value chemicals and pharmaceuticals in Flow Reactors (Unpublished). In: 5th IMTB. Cavtat, Croatia.

This list was generated on Sat Sep 25 09:42:21 2021 CEST.
  • Member of the Royal Society of Chemistry
  • Member of the American Chemical Society
  • Member of the Swiss Chemical Society
  • Member of the Flow Chemistry Society

 

 

 

News & Social Media